Description
TAMRA Desthiobiotin Azide is a trifunctional probe that allows for recovery of avidin-bound protein complexes in affinity-based assays. Desthiobiotinylated proteins can be eluted readily and specifically from avidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.